Join the Meeting Place for Moms!
Talk to other moms, share advice, and have fun!

(minimum 6 characters)

Natural Birth & Parenting Natural Birth & Parenting

Engineered E. coli the New Vaccine Adjuvant?

Posted by   + Show Post

The dirty little secret for making better vaccines

Posted by Daniel Oppenheimer-U. Texas on Monday, January 21, 2013

U. TEXAS-AUSTIN (US) — A menu of 61 new strains of genetically engineered bacteria may mean better vaccines for diseases like flu, whooping cough, cholera, and HPV.

The strains of E. coli are part of a new class of biological “adjuvants”—substances added to vaccines to boost the human immune response—that are poised to transform vaccine design, researchers say.

“For 70 years the only adjuvants being used were aluminum salts,” says Stephen Trent, associate professor of biology at the University of Texas at Austin. “They worked, but we didn’t fully understand why, and there were limitations. Then four years ago the first biological adjuvant was approved by the Food and Drug Administration. I think what we’re doing is a step forward from that. It’s going to allow us to design vaccines in a much more intentional way.”

Adjuvants were discovered in the early years of commercial vaccine production, when it was noticed that batches of vaccine that were accidentally contaminated often seemed to be more effective than those that were pure.

“They’re called the ‘dirty little secret’ of immunology,” Trent says. “If the vials were dirty, they elicited a better immune response.”

What researchers eventually realized was that they could produce a one-two punch by intentionally adding their own dirt (adjuvant) to the mix. The main ingredient of the vaccine, which was a killed or inactivated version of the bacteria or virus that the vaccine was meant to protect against, did what it was supposed to do. It “taught” the body’s immune system to recognize it and produce antibodies in response to it.

The adjuvant amplifies that response by triggering a more general alarm, which puts more agents of the immune system in circulation in the bloodstream, where they can then learn to recognize the key antigen. The result is an immune system more heavily armed to fight the virus or bacteria when it encounters it in the future.

For about 70 years the adjuvant of choice, in nearly every vaccine worldwide, was an aluminum salt. Then in 2009, the FDA approved a new vaccine for human papillomavirus (HPV). It included a new kind of adjuvant that’s a modified version of an endotoxin molecule.

These molecules, which can be dangerous, appear on the cell surface of a wide range of bacteria. As a result, humans have evolved over millions of years to detect and respond to them quickly. They trigger an immediate red alert.

“In some of its forms an endotoxin can kill you,” Trent says. “But the adjuvant, which is called MPL, is a very small, carefully modified piece of it, so it’s able to trigger the immune response without overdoing it.”

What Trent and his colleagues have done is expand on that basic premise, according to the study published in Proceedings of the National Academy of Sciences. Rather than just work with an inert piece of endotoxin, they’ve engineered E. coli bacteria to express the endotoxin in many configurations on the cell surface.

“These 61 E. coli strains each have a different profile on their surface,” says Brittany Needham, a doctoral student in Trent’s lab and the first author on the paper. “In every case the surface structure of the endotoxin is safe, but it will interact with the immune system in a range of ways. Suddenly we have a huge potential menu of adjuvants to test out with different kinds of vaccines.”

One form might work better with cholera vaccine, another with pertussis (whooping cough), and another with a future HIV vaccine. Trent, Needham, and their colleagues should be able to fine-tune the adjuvants with increasing precision as more E. coli strains are engineered and tested, and as their understanding of how they interact with the immune system deepens.

“I think we’re at the dawn of a new age of vaccine design,” Trent says. “For a long time vaccinology was really a trial-and-error field. It was a black box. We knew certain things worked. We knew certain vaccines had certain side effects. But we didn’t entirely know why. Now that’s changing.”

An additional advantage of their system is that the E. coli can be engineered to express key viral and bacterial antigens along with the endotoxin. A single cell could deliver both parts of the one-two punch, or even a one-two-three punch, if antigens from multiple diseases were expressed in a single E. coli.”It makes possible a vaccine that provides protection from multiple pathogens at the same time,” Trent says.

The researchers are working on a second round of designer E. coli. They have also filed a provisional patent on their system and are working with the university to find a corporate partner to pay for clinical trials.

“This is ready to go,” Trent says “I can’t predict whether it will actually make it to the market. But it’s very similar to the adjuvant that has already been approved, and my instinct is that if a company will undertake to do the trials, it will get approved. A company could call us tomorrow, we could send them a strain, and they could start working.”



source including link to original study

by on Jan. 25, 2013 at 3:10 PM
Replies (11-20):
ruby_jewel_04
by on Jan. 26, 2013 at 4:05 AM
Pretty much what I was thinking. Who in their right mind would put this in their body?!


Quoting mrsrobin:

Uh....



Everyone ready for a zombie apocalypse?

Posted on CafeMom Mobile
Mrs.Salz
by Platinum Member on Jan. 26, 2013 at 11:06 AM
1 mom liked this

Scary!! It amazing what people are ok with when a doctor or scientist tells them it's ok!

Quoting somuchlove4U:

Sad thing is, I posted this on my facebook. A friend of mine said, they explained it pretty well, I see no problem with it.
I didn't know how to respond.


jconney80
by Group Mod on Jan. 26, 2013 at 10:02 PM
Lol right

Quoting jellyphish:

Umm... April Fools?

This can't be for real. How can anyone read this info and think its a good idea?!
Posted on CafeMom Mobile
Mitzi31
by on Jan. 27, 2013 at 7:55 AM

This...

“I think we’re at the dawn of a new age of vaccine design,” Trent says. “For a long time vaccinology was really a trial-and-error field. It was a black box. We knew certain things worked. We knew certain vaccines had certain side effects. But we didn’t entirely know why. Now that’s changing.”

And docs can't understand why we as parents don't want to give our children vaccines? They are admitting they had no friggin idea what they were doing?? I don't want MY child to be a Guinea pig in the vaccine experiment to find what works.

Mitzi31
by on Jan. 27, 2013 at 7:59 AM

This is NOT natural. Our bodies are meant for the diseases to go into the mouth, eyes ears things like that and we fight them off. They are being injected into our blood stream and that's NOT how it supposed to happen. Our bodies aren't made for that. If someone wants to argue that they are giving vaccines for the "greater good" then that's fine but you can't tell me it's cause you love your child and you want what's best for your child. MOST of these diseases are very rare that a child will get and if there's a compromised immune system and a real need for it it's one thing... but to give an otherwise heatlhy child this... disease and all the crap that goes a long with it is NOT healthy.

Not totally knocking people who get vaccines cause most do it cause the docs say it's best and we USED to be able to trust our doctors. But if you really do the research I don't see how you can be ok with your child being injected with all this stuff! Especially at 2 months of age!

HeatherRainbow
by on Jan. 27, 2013 at 9:40 AM

Gives new potential to religious vaccines... Last I knew, humans were not Gods or Goddesses, so therefore, since this is manipulating of bacteria and life, ie, playing God, then it goes against my religion.

Anyone who would try this just doesn't understand the horror of what it could do, not only in their own bodies, but in a biological warfare sort of situation. Antibiotics are creating super bugs, what the hell... don't they see this has the potential to do something along these same lines????

Juniper53098
by June on Feb. 3, 2013 at 12:55 AM

Since they started using this in the HPV vaccine, wonder if it's the cause of the HORRENDOUS side effects that they won't admit to....

kiki2382
by on Feb. 3, 2013 at 10:00 AM
What are the side effects? I haven't done my vax research yet.

Quoting Juniper53098:

Since they started using this in the HPV vaccine, wonder if it's the cause of the HORRENDOUS side effects that they won't admit to....

Posted on the NEW CafeMom Mobile
Juniper53098
by June on Feb. 3, 2013 at 11:14 AM

This article lists the official side effects and also talks about the side effects that have been reported but not acknowledged by the CDC.

http://www.ehow.com/about_5172123_hpv-vaccine-long_term-side-effects.html

Quoting kiki2382:

What are the side effects? I haven't done my vax research yet.

Quoting Juniper53098:

Since they started using this in the HPV vaccine, wonder if it's the cause of the HORRENDOUS side effects that they won't admit to....


kiki2382
by on Feb. 3, 2013 at 11:32 AM
Thanks!

Quoting Juniper53098:

This article lists the official side effects and also talks about the side effects that have been reported but not acknowledged by the CDC.

http://www.ehow.com/about_5172123_hpv-vaccine-long_term-side-effects.html

Quoting kiki2382:

What are the side effects? I haven't done my vax research yet.



Quoting Juniper53098:

Since they started using this in the HPV vaccine, wonder if it's the cause of the HORRENDOUS side effects that they won't admit to....


Posted on the NEW CafeMom Mobile
Add your quick reply below:
You must be a member to reply to this post.
Join the Meeting Place for Moms!
Talk to other moms, share advice, and have fun!

(minimum 6 characters)